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cIAP2 Recombinant Rabbit mAb (S-2483-8)

Baculoviral IAP repeat-containing protein 3,Apoptosis inhibitor 2 (API2),Cellular inhibitor of apoptosis 2,IAP homolog C,Inhibitor of apoptosis protein 1 (hIAP-1,hIAP1),RING finger protein 49,API2,MIHC,RNF49,BIRC3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6458

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

c-IAP2
分子别名

Baculoviral IAP repeat-containing protein 3; Apoptosis inhibitor 2 (API2); Cellular inhibitor of apoptosis 2; IAP homolog C; Inhibitor of apoptosis protein 1 (hIAP-1; hIAP1); RING finger protein 49; API2; MIHC; RNF49; BIRC3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13489
克隆号

S-2483-8
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

Raji, Ramos, Daudi, F9, mouse spleen, mouse testis, rat testis, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms, Rt, Mk
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu, Ms, Rt

背景介绍

Cellular inhibitor of apoptosis protein 2 (c-IAP2, gene BIRC3) is a 618-residue member of the IAP family containing three N-terminal baculoviral IAP repeat (BIR) domains that bind caspases, a central caspase-recruitment domain (CARD) that auto-inhibits its own ubiquitin-ligase activity, and a C-terminal RING domain that endows potent E3 ubiquitin-ligase activity; through this enzymatic function c-IAP2 polyubiquitinates receptor-interacting protein 1 (RIP1) and NF-κB-inducing kinase (NIK), thereby driving canonical NF-κB prosurvival signaling while suppressing non-canonical NF-κB activation, controls TNF-α-mediated apoptosis, regulates innate immunity downstream of Toll-like and NOD-like receptors, and is frequently amplified or over-expressed in multiple cancers where its anti-apoptotic activity contributes to chemoresistance, making it a prime target for small-molecule Smac mimetics that induce its autoubiquitination and proteasomal degradation.

免疫印迹
- WB result of cIAP2 Recombinant Rabbit mAb
  Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Raji whole cell lysate 20 µg
  Lane 2: Ramos whole cell lysate 20 µg
  Lane 3: Daudi whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
- WB result of cIAP2 Recombinant Rabbit mAb
  Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: F9 whole cell lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Lane 3: mouse testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
- WB result of cIAP2 Recombinant Rabbit mAb
  Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
- WB result of cIAP2 Recombinant Rabbit mAb
  Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 70 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of cIAP2 in tumor tissue.
- Expression of cIAP2 in human tissue.
- Expression of cIAP2 in mouse & rat tissue.