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Rabbit抗原名称
Calnexin分子别名
IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
P27824抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, U-2 OS, MCF7, THP-1, SH-SY5Y, HepG2, LNCaP纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:250 Hu ICC 1:500 Hu
Calnexin is an integral endoplasmic reticulum (ER) type I transmembrane lectin chaperone that transiently binds newly synthesized monoglucosylated N-glycoproteins through its luminal carbohydrate-binding domain, coordinates sequential folding events with the oxidoreductase ERp57 to form disulfide bonds, retains incompletely folded or misassembled polypeptides in the ER via a cytosolic phosphorylation-regulated retrieval motif that interacts with the Sec61 translocon, and thereby serves as a central quality-control checkpoint that prevents aggregation and premature trafficking, while also participating in ER calcium homeostasis and cellular stress responses such as the unfolded protein response (UPR).
免疫印迹
WB result of Calnexin Rabbit pAb
Primary antibody: Calnexin Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: THP-1 whole cell lysate 20 µg
Lane 5: SH-SY5Y whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Lane 7: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Calnexin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Calnexin in tumor tissue.
Expression of Calnexin in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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