Bax Recombinant Rabbit mAb (S-2603-124)

Apoptosis regulator BAX

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6456

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

BAX
分子别名

Apoptosis regulator BAX
免疫原

Synthetic Peptide
细胞定位

Mitochondrion, Cytoplasm, Nucleus
Accession

Q07813
克隆号

S-2603-124
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P ? ,
WB
反应种属 ?

Hu, Ms, Mk
阳性样本

HEK-293, NIH/3T3, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Mk
IHC-P	1:1000-1:5000	Hu, Ms

背景介绍

Bax is a pro-apoptotic member of the Bcl-2 protein family that localizes to the cytosol of healthy cells but, upon activation by developmental death cues or intracellular stress such as DNA damage, undergoes conformational change and translocates to the outer mitochondrial membrane where it oligomerizes to form pores that permeabilize the membrane, thereby releasing cytochrome c and other apoptogenic factors into the cytosol to trigger the caspase cascade and ultimately apoptosis.

免疫印迹
- WB result of Bax Recombinant Rabbit mAb
  Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HEK-293 whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 21 kDa
  Observed MW: 22 kDa
- WB result of Bax Recombinant Rabbit mAb
  Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 21 kDa
  Observed MW: 22 kDa
- WB result of Bax Recombinant Rabbit mAb
  Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 21 kDa
  Observed MW: 22 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of Bax in tumor tissue.
- Expression of Bax in human tissue.
- Expression of Bax in mouse & rat tissue.