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Rabbit抗原名称
AHR分子别名
Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76 (bHLHe76); BHLHE76免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P35869克隆号
S-2370-150抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
A204, HepG2, A431, MCF7, PC-3, C2C12, mouse testis, rat liver, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu ChIP 1:20-1:50 Hu
The aryl hydrocarbon receptor (AHR) is an evolutionarily conserved, ligand-activated transcription factor that resides primarily in the cytoplasm as part of an HSP90-containing multiprotein complex; upon binding planar aromatic ligands—ranging from environmental pollutants such as dioxins and polycyclic aromatic hydrocarbons to endogenous or dietary molecules like tryptophan photoproducts and flavonoids—it translocates to the nucleus, heterodimerizes with its partner ARNT (HIF-1β), and binds xenobiotic response elements (XRE/DRE) to drive transcription of genes involved in xenobiotic metabolism (e.g., CYP1A1), but it also exerts broad non-genomic and non-canonical actions by crosstalking with NF-κB, Wnt, MAPK, and hypoxia signaling pathways, thereby modulating immune cell differentiation, inflammatory cytokine production, antioxidant responses, cell cycle checkpoints, and even osteoclastogenesis, placing AHR at the hub of environmental sensing, metabolic homeostasis, immune tolerance, and pathologies including autoimmunity, cancer, osteoporosis, and premature aging.
免疫印迹
WB result of AHR Recombinant Rabbit mAb
Primary antibody: AHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A204 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 96 kDaWB result of AHR Recombinant Rabbit mAb
Primary antibody: AHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 96 kDaWB result of AHR Recombinant Rabbit mAb
Primary antibody: AHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 96 kDaWB result of AHR Recombinant Rabbit mAb
Primary antibody: AHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 96 kDa
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-AHR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HepG2 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
Parallel reactions used AHR Recombinant Rabbit mAb (S-2370-150) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of CYP1A1, NFE2L2 and SAT-α in AHR Recombinant
Rabbit mAb (S-2370-150)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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