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Stat5 Recombinant Rabbit mAb (S-2823-24)

Signal transducer and activator of transcription 5B,STAT5B

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6450
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Stat5

  • 分子别名

    Signal transducer and activator of transcription 5B; STAT5B

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P51692

  • 克隆号

    S-2823-24

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    K562, HDLM-2, Raji, HeLa, Jurkat, A20, mouse heart, PC-12, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
    IHC-P 1:50-1:200 Hu, Ms, Rt
    ChIP 1:20-1:50 Hu
背景介绍

  • Stat5 (Signal Transducer and Activator of Transcription 5) is a latent cytoplasmic transcription factor that becomes phosphorylated on a conserved tyrosine (Tyr694 in human STAT5a) by activated Janus kinases—primarily JAK2—following cytokine (e.g., IL-2, IL-3, GM-CSF, GH, PRL, EPO, TPO) or oncogenic tyrosine-kinase receptor (e.g., BCR-ABL, FLT3-ITD, JAK2V617F) signaling, whereupon it forms parallel homo- or heterodimers, translocates into the nucleus, binds the consensus DNA motif TTCNNNGAA within enhancers/promoters of target genes like CISH, BCL2, MYC, CCND1, PIM1 and SOCS proteins, and thereby orchestrates proliferation, survival, self-renewal and differentiation programs in hematopoietic, mammary and other cell types; persistent Stat5 activation is a driver of myeloproliferative neoplasms, acute leukemias and breast cancers, making its upstream kinases and the Stat5 SH2 or DNA-binding domains attractive therapeutic targets.

  • 免疫印迹

    • WB result of Stat5 Recombinant Rabbit mAb
      Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-3 whole cell lysate 20 µg
      Lane 2: K562 whole cell lysate 20 µg
      Lane 3: HDLM-2 whole cell lysate 20 µg
      Lane 4: Raji whole cell lysate 20 µg
      Lane 5: HeLa whole cell lysate 20 µg
      Lane 6: Jurkat whole cell lysate 20 µg
      Negative control: PC-3 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 90 kDa
      Observed MW: 90 kDa

    • WB result of Stat5 Recombinant Rabbit mAb
      Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A20 whole cell lysate 20 µg
      Lane 2: mouse heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 90 kDa
      Observed MW: 90 kDa

    • WB result of Stat5 Recombinant Rabbit mAb
      Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 90 kDa
      Observed MW: 90 kDa

    • WB result of Stat5 Recombinant Rabbit mAb
      Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 90 kDa
      Observed MW: 90 kDa

    • WB result of Stat5 Recombinant Rabbit mAb
      Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Human stat5a full length recombinant protein 10 ng
      Lane 2: Human stat5b full length recombinant protein 10 ng
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 90 kDa
      Observed MW: 100 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-stat5 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-stat5 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on K562 cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Stat5 Recombinant Rabbit mAb (S-2823-24) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR showed the enrichment of CISH and RPL30 in Stat5 Recombinant Rabbit mAb
      (S-2823-24)-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of stat5 in tumor tissue.

    • Expression of stat5 in human tissue.

    • Expression of stat5 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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