分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
CD328分子别名
Sialic acid-binding Ig-like lectin 7; Siglec-7; Adhesion inhibitory receptor molecule 1 (AIRM-1); CDw328; D-siglec; QA79 membrane protein; p75; CD328; AIRM1; SIGLEC7免疫原
Recombinant Protein细胞定位
MembraneAccession
Q9Y286克隆号
S-2116-168抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
U937, RPMI-8226纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CD328, also known as Siglec-7, is a 467-amino-acid inhibitory receptor of the CD33-related Siglec family that displays a single N-terminal V-set immunoglobulin domain for binding α2,3- and α2,6-linked sialic acids, followed by two C2-set Ig domains, a transmembrane segment and a cytoplasmic tail containing two ITIMs; expressed predominantly on NK cells, CD8⁺ T cells and monocytes, it recruits SHP-1/2 phosphatases upon engagement of sialylated glycans, thereby dampening cytotoxicity, cytokine release and cellular activation to help maintain immune tolerance.
免疫印迹
WB result of Siglec7 Recombinant Rabbit mAb
Primary antibody: Siglec7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: U937 whole cell lysate 20 µg
Lane 4: RPMI-8226 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; PANC-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 60-100 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)