SCARB2/LIMPII Recombinant Rabbit mAb (S-2847-21)
Lysosome membrane protein 2,85 kDa lysosomal membrane sialoglycoprotein (LGP85),CD36 antigen-like 2,Lysosome membrane protein II (LIMP II),Scavenger receptor class B member 2,CD36,CD36L2,LIMP2
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宿主来源
Rabbit抗原名称
SCARB2/LIMPII分子别名
Lysosome membrane protein 2; 85 kDa lysosomal membrane sialoglycoprotein (LGP85); CD36 antigen-like 2; Lysosome membrane protein II (LIMP II); Scavenger receptor class B member 2; CD36; CD36L2; LIMP2免疫原
Synthetic Peptide细胞定位
LysosomeAccession
Q14108克隆号
S-2847-21抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
SH-SY5Y, HT-29, HeLa, HepG2, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk IHC-P 1:1000 Hu ICC 1:100 Hu
SCARB2/LIMPII, also called lysosomal integral membrane protein-2 (LIMP-2), is an ~85 kDa heavily glycosylated type-III multi-pass membrane protein that resides predominantly in the limiting membranes of lysosomes and late endosomes in every mammalian tissue, where it functions as the mannose-6-phosphate-independent trafficking receptor that escorts the lipid-catabolizing enzyme β-glucocerebrosidase (GBA) from the Golgi to the lysosome lumen, and also serves as the primary cell-surface receptor for enterovirus 71 and several coxsackieviruses that cause hand-foot-and-mouth disease, while genetic loss-of-function mutations in SCARB2 produce the autosomal-recessive “action myoclonus–renal failure syndrome” characterized by progressive myoclonic epilepsy, renal glomerulosclerosis, deafness and peripheral neuropathy.
免疫印迹
WB result of SCARB2/LIMPII Recombinant Rabbit mAb
Primary antibody: SCARB2/LIMPII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDaWB result of SCARB2/LIMPII Recombinant Rabbit mAb
Primary antibody: SCARB2/LIMPII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SCARB2/LIMPII antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SH-SY5Y cells. Anti-SCARB2/LIMPII antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SCARB2/LIMPII in tumor tissue.
Expression of SCARB2/LIMPII in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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