分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
PLK1分子别名
Serine/threonine-protein kinase PLK1; Polo-like kinase 1 (PLK-1); Serine/threonine-protein kinase 13 (STPK13); PLK免疫原
Synthetic Peptide细胞定位
Cytoskeleton, Nucleus, CytoplasmAccession
P53350克隆号
S-2835-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
Ramos, HepG2, HeLa, HEK-293, Jurkat, mouse testis, PC-12, rat testis, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu ICFCM 1:50 Hu
PLK1 (Polo-like kinase 1) is a 603-residue serine/threonine kinase that peaks in late G2/M where it docks to phosphorylated Bub1 and the PBD-binding p-T78 motif on its own T-loop to auto-activate, then successively phosphorylates Cdc25C, Cyclin B1, Wee1, APC/C-Cdc20, and myriad kinetochore proteins to drive centrosome maturation, bipolar spindle assembly, chromosome alignment, the spindle-assembly and decatenation checkpoints, and timely mitotic exit, while its deregulated overexpression in cancers creates chromosomal instability and chemoresistance, making it a high-priority drug target whose ATP-competitive inhibitors (BI 2536, volasertib) are already in oncology trials.
免疫印迹
WB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDaWB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDaWB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDaWB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling PLK1 antibody at 1/50 dilution (1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-PLK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)