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Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb (S-3537)

Mitogen-activated protein kinase 8,MAP kinase 8,MAPK 8,JNK-46,Stress-activated protein kinase 1c (SAPK1c),Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,JNK1,PRKM8,SAPK1,SAPK1C,MAPK8

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6444
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-SAPK/JNK (Thr183/Tyr185)

  • 分子别名

    Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; JNK1; PRKM8; SAPK1; SAPK1C; MAPK8

  • 细胞定位

    Nucleus

  • Accession

    P45983

  • 克隆号

    S-3537

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Dm, Sc

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
背景介绍

  • Phospho-SAPK/JNK (Thr183/Tyr185) refers to the activated form of Stress-Activated Protein Kinase/c-Jun N-terminal Kinase (SAPK/JNK) that is simultaneously phosphorylated at its threonine 183 (Thr183) and tyrosine 185 (Tyr185) residues. As a key member of the Mitogen-Activated Protein Kinase (MAPK) family, it is primarily activated by environmental stress signals such as ultraviolet radiation, gamma rays, inflammatory cytokines, and oxidative stress. Its activation depends on the dual phosphorylation of these two critical residues, Thr183 and Tyr185, by upstream kinases MKK4 or MKK7. This modification acts like a "molecular switch," altering JNK's conformation from an inactive to an active state. Once activated, Phospho-SAPK/JNK (Thr183/Tyr185) dimerizes and translocates into the nucleus. Inside the nucleus, it phosphorylates transcription factors like c-Jun and ATF-2, thereby regulating the expression of a suite of genes involved in cellular stress response, apoptosis, inflammation, and even proliferation and differentiation. Consequently, this specific phosphorylated form of the protein is not only a direct marker for JNK pathway activation, but its dysregulated activity is also closely linked to various pathological processes, including neurodegenerative diseases, inflammatory conditions, and cancer, making it a significant target in life science research and the exploration of disease mechanisms.

  • 免疫印迹

    • WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HEK-293 whole cell lysate 20 µg
      Lane 2: HEK-293 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 40, 52 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 40, 52 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 40, 52 kDa
      This blot was developed with high sensitivity substrate

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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