Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb (S-3551)
Ephrin type-A receptor 3,EPH-like kinase 4 (EK4,hEK4),HEK (Human embryo kinase),Tyrosine-protein kinase TYRO4,Tyrosine-protein kinase receptor ETK1 (Eph-like tyrosine kinase 1),ETK,ETK1,HEK,TYRO4,EPHA3
分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Phospho-EphA3 (Tyr779)分子别名
Ephrin type-A receptor 3; EPH-like kinase 4 (EK4; hEK4); HEK (Human embryo kinase); Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1 (Eph-like tyrosine kinase 1); ETK; ETK1; HEK; TYRO4; EPHA3细胞定位
Secreted, Cell membraneAccession
P29320克隆号
S-3551抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Phospho-EphA3 (Tyr779) is the ligand-triggered, autophosphorylated form of the receptor tyrosine kinase EphA3 that localizes within the kinase domain; its phosphorylation is essential for full receptor activation, creates a docking site for the SH2-domain adaptor CrkII, and thereby couples EphA3 to downstream RhoA/ROCK signaling to regulate actomyosin dynamics, cell adhesion, migration and neurite retraction.
免疫印迹
WB result of Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated MDA-MB-231 whole cell lysate 20 µg
Lane 2: MDA-MB-231 starve overnight, then treated with 100 ng/ml Ephrin-A for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 120 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
评论(0)