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Rabbit抗原名称
MX1分子别名
Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78 (IFI-78K); Interferon-regulated resistance GTP-binding protein MxA; Myxoma resistance protein 1; Myxovirus resistance protein 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Endoplasmic reticulumAccession
P20591抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
MX1 (Myxovirus resistance protein 1) is an interferon-inducible dynamin-like large GTPase that constitutes a frontline intracellular defense against a broad spectrum of RNA and DNA viruses by recognizing viral nucleocapsids, oligomerizing into helical assemblies driven by GTP binding and hydrolysis, and sequestering or mis-sorting these capsids away from replication sites, thereby blocking viral transcription and genome amplification; the 662-aa human protein (also termed MxA) contains an N-terminal GTPase domain connected via a bundle-signalling element to a C-terminal stalk that together mediate self-assembly, while a disordered L4 loop and a unique N-terminal segment dictate its predominantly cytoplasmic localization, determine its extensive antiviral breadth including influenza A, vesicular stomatitis, bunyaviruses and HBV, and explain why polymorphisms or truncations in MX1 can abolish this activity and increase host susceptibility to infection.
免疫印迹
WB result of MX1 Rabbit pAb
Primary antibody: MX1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml hIFN-α1 for 16 hours whole cell lysate 20 µg
Lane 3: untreated A549 whole cell lysate 20 µg
Lane 4: A549 treated with 10 ng/ml hIFN-α1 for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells treated with hIFN-α1(10ng/ml, 16h) (top panel) and untreated HeLa cells (below panel). Anti- MX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MX1 in tumor tissue.
Expression of MX1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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