分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
LY6E/SCA-2分子别名
Lymphocyte antigen 6E; Ly-6E; Retinoic acid-induced gene E protein (RIG-E); Stem cell antigen 2 (SCA-2); Thymic shared antigen 1 (TSA-1); 9804; RIGE; SCA2; TSA1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q16553克隆号
S-2681-56抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
T-47D, HT-29, HCT 116, SW480, SW620, HEK-293纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:500 Hu
LY6E/SCA-2 is a ~14 kDa glycosylphosphatidylinositol-anchored cell-surface protein of the Ly6/uPAR superfamily that is constitutively expressed in thymus, spleen, placenta and many epithelia and is strongly up-regulated by type I interferons; it functions as a context-dependent modulator of immunity and oncogenesis by tuning T-cell receptor/CD3ζ phosphorylation, enhancing HIV-1 and other viral replication while conversely restricting coronavirus entry via spike-protein interference, and its over-expression in multiple tumors promotes immune escape, metastasis and chemoresistance, making it a candidate prognostic marker and therapeutic target.
免疫印迹
WB result of LY6E/SCA-2 Recombinant Rabbit mAb
Primary antibody: LY6E/SCA-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Lane 5: SW620 whole cell lysate 20 µg
Lane 6: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 13 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in T-47D cells. Anti-LY6E/SCA-2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)