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Rabbit抗原名称
Lipocalin-2/NGAL分子别名
Neutrophil gelatinase-associated lipocalin; Lipocalin-2; Oncogene 24p3 (24p3); SV-40-induced 24p3 protein; Siderocalin LCN2; p25; Lcn2免疫原
Synthetic Peptide细胞定位
SecretedAccession
P11672克隆号
S-2280-129抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse spleen, mouse bone marrow, mouse lung, rat spleen ,rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:2000 Ms, Rt IF 1:500 Ms, Rt
Lipocalin-2/NGAL is a 25-kDa secreted glycoprotein of the lipocalin superfamily that forms an 8-stranded β-barrel calyx able to bind and transport ferric siderophore complexes, thereby acting as a bacteriostatic innate immune factor that starves bacteria of iron while simultaneously delivering iron to mammalian cells via the megalin/24p3R receptor, and is rapidly induced by inflammatory, hypoxic or nephrotoxic stress in neutrophils, macrophages and epithelia, circulates as monomer, homodimer or MMP-9–linked heterodimer, functions as a sensitive biomarker of acute and chronic kidney injury, modulates cell survival, differentiation, epithelial-to-mesenchymal transition and oncogenesis, and is transcriptionally up-regulated through NF-κB, ERK and JAK-STAT pathways in metabolic disorders such as obesity and insulin resistance.
免疫印迹
WB result of Lipocalin-2/NGAL Recombinant Rabbit mAb
Primary antibody: Lipocalin-2/NGAL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: mouse bone marrow lysate 20 µg
Lane 5: mouse lung lysate 20 µg
Negative control: mouse heart lysate; mouse brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDaWB result of Lipocalin-2/NGAL Recombinant Rabbit mAb
Primary antibody: Lipocalin-2/NGAL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat lung lysate 20 µg
Negative control: rat heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse spleen. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse lung. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded mouse brain. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat spleen. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat lung. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded rat brain. Anti- Lipocalin-2/NGAL antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Lipocaliln-2/NGAL in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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