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Rabbit抗原名称
LIN28A分子别名
Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; CSDD1; LIN28; ZCCHC1免疫原
Recombinant Protein细胞定位
Nucleus, Endoplasmic reticulum, CytoplasmAccession
Q9H9Z2抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
T-47D, NCCIT, JAR, NTERA-2, F9纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms IHC-P 1:500-1:1000 Hu, Ms ICC 1:500 Hu
LIN28A is an evolutionarily-conserved RNA-binding protein that harbors a cold-shock domain and two CCHC-type zinc knuckles, enabling it to dock onto specific motifs (e.g., UGAU loops) in mRNAs and micro-RNA precursors; by blocking the biogenesis of the tumor-suppressive let-7 family and by directly stabilizing or enhancing translation of metabolic, hypoxic and pluripotency transcripts (including HIF1α, IGF2BP3 and OCT4 targets), LIN28A sustains pluripotency, drives aerobic glycolysis, fosters embryonic and adult tissue regeneration, and when re-activated in cancers promotes stemness, angiogenesis, epithelial-to-mesenchymal transition, chemoresistance and poor patient prognosis.
免疫印迹
WB result of LIN28A Rabbit pAb
Primary antibody: LIN28A Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Lane 5: JAR whole cell lysate 20 µg
Lane 6: NTERA-2 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 24 kDaWB result of LIN28A Rabbit pAb
Primary antibody: LIN28A Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: F9 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 24 kDa
免疫组化
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-LIN28A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-LIN28A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human prostatic cancer. Anti-LIN28A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-LIN28A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-LIN28A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in JAR cells (top panel) and negative staining in HeLa cells (below panel). Anti- LIN28A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of LIN28A in tumor tissue.
Expression of LIN28A in human tissue.
Expression of LIN28A in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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