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IGF2BP1 Recombinant Rabbit mAb (S-2947-19)

Insulin-like growth factor 2 mRNA-binding protein 1,IGF2 mRNA-binding protein 1,IMP-1,IMP1,Coding region determinant-binding protein (CRD-BP),IGF-II mRNA-binding protein 1,VICKZ family member 1,Zipcode-binding protein 1 (ZBP-1),CRDBP,VICKZ1,ZBP1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6432

规格

25μl100μl

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

IGF2BP1
分子别名

Insulin-like growth factor 2 mRNA-binding protein 1; IGF2 mRNA-binding protein 1; IMP-1; IMP1; Coding region determinant-binding protein (CRD-BP); IGF-II mRNA-binding protein 1; VICKZ family member 1; Zipcode-binding protein 1 (ZBP-1); CRDBP; VICKZ1; ZBP1
免疫原

Recombinant Protein
细胞定位

Cytoplasm
Accession

Q9NZI8
克隆号

S-2947-19
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

Caco-2, Jurkat, K562, HeLa, 293T, NIH/3T3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:1000	Hu
ICC	1:500	Hu

背景介绍

IGF2BP1 (Insulin-like growth factor-2 mRNA-binding protein 1) is a 63-kDa oncofetal RNA-binding protein that contains four KH and two RRM domains and preferentially binds thousands of coding and non-coding transcripts—most prominently IGF2, MYC, KRAS, MDR1, and β-TRCP1 mRNAs—via conserved CAUH (H = A/C/U) motifs in their 3′-UTRs, thereby protecting them from endonucleolytic cleavage and miRNA-mediated silencing, stabilizing the transcripts, enhancing their translation, and consequently driving cell growth, self-renewal, migration, and chemoresistance in embryonic, stem, and cancer cells; the protein dynamically shuttles between the nucleus and cytoplasm in a methylation- and phosphorylation-regulated manner, localizes to stress granules and P-bodies, and its elevated expression in hepatocellular, colorectal, ovarian, pancreatic, and triple-negative breast carcinomas correlates with advanced stage, metastasis, and poor prognosis, making IGF2BP1 a promising prognostic biomarker and therapeutic target.

免疫印迹
- WB result of IGF2BP1 Recombinant Rabbit mAb
  Primary antibody: IGF2BP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: U-2 OS whole cell lysate 20 µg
  Lane 2: Caco-2 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: K562 whole cell lysate 20 µg
  Lane 5: HeLa whole cell lysate 20 µg
  Lane 6: 293T whole cell lysate 20 µg
  Negative control: U-2 OS whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 63 kDa
  Observed MW: 68 kDa
- WB result of IGF2BP1 Recombinant Rabbit mAb
  Primary antibody: IGF2BP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 63 kDa
  Observed MW: 68 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-IGF2BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-IGF2BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-IGF2BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Caco-2 cells (top panel) and negative staining in U2-OS cells (below panel). Anti- IGF2BP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of IGF2BP1 in tumor tissue.
- Expression of IGF2BP1 in human tissue.