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GCLM Recombinant Rabbit mAb (S-2209-19)

Glutamate--cysteine ligase regulatory subunit,GCS light chain,Gamma-ECS regulatory subunit,Gamma-glutamylcysteine synthetase regulatory subunit,Glutamate--cysteine ligase modifier subunit,GLCLR

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6431

规格

25μl100μl

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

GCLM
分子别名

Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit; GLCLR
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P48507
克隆号

S-2209-19
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HeLa, A431, HepG2, K562, A549, NIH/3T3, mouse liver, PC-12, rat liver, COS-7
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms, Rt, Mk
IHC-P 1:200-1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:200-1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

GCLM (glutamate-cysteine ligase modifier subunit) is the regulatory component of glutamate-cysteine ligase (GCL), the rate-limiting enzyme in glutathione biosynthesis; by forming a heterodimer with the catalytic GCLC subunit, GCLM increases the enzyme’s affinity for glutamate and ATP, raises its Ki for glutathione feedback inhibition, and thus accelerates production of the antioxidant glutathione that maintains cellular redox balance and protects against oxidative stress.

免疫印迹
- WB result of GCLM Recombinant Rabbit mAb
  Primary antibody: GCLM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: K562whole cell lysate 20 µg
  Lane 5: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 34 kDa
- WB result of GCLM Recombinant Rabbit mAb
  Primary antibody: GCLM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 34 kDa
- WB result of GCLM Recombinant Rabbit mAb
  Primary antibody: GCLM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 34 kDa
- WB result of GCLM Recombinant Rabbit mAb
  Primary antibody: GCLM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 34 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti-GCLM antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-GCLM antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-GCLM antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-GCLM antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-GCLM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-GCLM antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-GCLM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of GCLM in tumor tissue.
- Expression of GCLM in human tissue.
- Expression of GCLM in mouse & rat tissue.