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Rabbit抗原名称
CD61分子别名
Integrin beta-3; Platelet membrane glycoprotein IIIa (GPIIIa); GP3A; ITGB3免疫原
Synthetic Peptide细胞定位
Cell projection, Cell membraneAccession
P05106克隆号
S-2515-6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
U-87 MG, HUVEC, C2C12, RAW264.7, mouse spleen, COS-7, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:500-1:2000 Hu, Ms, Rt IF 1:500 Hu
CD61, also known as integrin beta-3 (ITGB3) or glycoprotein IIIa (gpIIIa), is a transmembrane protein encoded by the ITGB3 gene in humans. It is predominantly expressed on the surface of platelets and megakaryocytes. CD61 forms a crucial complex with CD41 (alphaIIb) to create the heterodimeric receptor gpIIb/IIIa, which is essential for platelet aggregation and thrombus formation. This receptor binds to fibrinogen, von Willebrand factor, and other extracellular matrix proteins, facilitating platelet adhesion and aggregation. In addition to its role in hemostasis, CD61 is also involved in various cellular processes, including cell adhesion, migration, and proliferation. It has been implicated in tumor progression, where it can influence tumor metabolism, immune microenvironment, and epithelial-to-mesenchymal transition.
免疫印迹
WB result of CD61 Recombinant Rabbit mAb
Primary antibody: CD61 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Low expression control: PC-3 whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 90 kDaWB result of CD61 Recombinant Rabbit mAb
Primary antibody: CD61 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 90 kDaWB result of CD61 Recombinant Rabbit mAb
Primary antibody: CD61 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 90 kDaWB result of CD61 Recombinant Rabbit mAb
Primary antibody: CD61 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-CD61 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-CD61 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD61 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD61 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-CD61 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD61 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD61 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human cardiac muscle. Anti-CD61 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human spleen. Anti-CD61 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CD61 in tumor tissue.
Expression of CD61 in human tissue.
Expression of CD61 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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