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Rabbit抗原名称
CaMKII alpha分子别名
Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMKA; KIAA0968; CAMK2A免疫原
Synthetic Peptide细胞定位
SynapseAccession
Q9UQM7克隆号
S-2451-54抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, U-87 MG, mouse brain, rat brain预测反应种属
(反应种属缩写表)Or, Ck, Pg, Xe, Bv, Zf, Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
CaMKII alpha is a major isoform of Ca²⁺/calmodulin-dependent protein kinase II, predominantly expressed in the mammalian central nervous system, with high abundance in brain regions associated with learning and memory, such as the hippocampus and cerebral cortex. This protein consists of multiple catalytic domains, regulatory domains, and an oligomerization domain, typically forming 12-mer homo- or hetero-oligomeric complexes. Its core function is to decode calcium signals: when intracellular Ca²⁺ levels rise, the Ca²⁺/calmodulin complex binds to CaMKII alpha, relieving autoinhibition and activating its kinase activity. This allows it to phosphorylate various substrates, including AMPA and NMDA receptors, thereby regulating synaptic plasticity and neuronal signaling. A key characteristic is its ability to undergo "autophosphorylation" – autophosphorylation at Thr286 enables CaMKII alpha to retain partial activity even after the Ca²⁺ signal dissipates. This mechanism is considered a molecular "memory switch," crucial for long-term potentiation (LTP) and the formation of learning and memory. Furthermore, dysregulated expression or altered activity of CaMKII alpha is closely associated with various neurological disorders, such as epilepsy and Alzheimer's disease.
免疫印迹
WB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDaWB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDaWB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CaMKII alpha in tumor tissue.
Expression of CaMKII alpha in human tissue.
Expression of CaMKII alpha in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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