APC6 Recombinant Rabbit mAb (S-2550-48)

Cell division cycle protein 16 homolog,Anaphase-promoting complex subunit 6 (APC6),Cyclosome subunit 6,Anapc6,Cdc16

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6424

规格

25μl100μl

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

APC6
分子别名

Cell division cycle protein 16 homolog; Anaphase-promoting complex subunit 6 (APC6); Cyclosome subunit 6; Anapc6; Cdc16
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus
Accession

Q8R349
克隆号

S-2550-48
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

NIH/3T3, mouse liver, C6, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1: 2000 Ms, Rt
IHC-P 1:100-1: 1000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000-1: 2000	Ms, Rt
IHC-P	1:100-1: 1000	Hu, Ms, Rt

背景介绍

APC6 (also known as CDC16) is a core scaffolding subunit of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase that governs cell cycle progression by targeting key regulatory proteins such as cyclins and securin for proteasomal degradation; APC6 contains multiple tetratricopeptide repeats (TPRs) that mediate protein-protein interactions essential for APC/C integrity and is stabilized by the small subunit CDC26, ensuring proper ubiquitin transfer and timely transition from metaphase to anaphase, thereby safeguarding genomic stability.

免疫印迹
- WB result of APC6 Recombinant Rabbit mAb
  Primary antibody: APC6 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 71 kDa
  Observed MW: 71 kDa
- WB result of APC6 Recombinant Rabbit mAb
  Primary antibody: APC6 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 71 kDa
  Observed MW: 71 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-APC6 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-APC6 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-APC6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-APC6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of APC6 in tumor tissue.
- Expression of APC6 in human tissue.
- Expression of APC6 in mouse & rat tissue.