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Rabbit抗原名称
Anterior Gradient 2分子别名
Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog; AG2; AGR2免疫原
Synthetic Peptide细胞定位
SecretedAccession
O95994抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HT-29, MCF7, Caco-2, COLO 205, A549, mouse stomach, rat stomach纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Anterior Gradient 2 (AGR2) is an ~20 kDa endoplasmic-reticulum–resident protein disulfide-isomerase that catalyzes disulfide-bond formation in nascent mucins and other secreted proteins, acts as a molecular chaperone to maintain ER proteostasis, and is highly expressed in mucosal epithelia; in cancers its overexpression is linked to aggressive growth, metastasis, chemoresistance and suppression of type-I interferon responses, while its loss triggers ER stress and the unfolded-protein response, and a secreted monomeric form detectable in serum and urine serves as a promising extracellular biomarker.
免疫印迹
WB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Lane 5: COLO 205 whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDaWB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDaWB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Anterior Gradient 2 in tumor tissue.
Expression of Anterior Gradient 2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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