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Rabbit抗原名称
Androgen Receptor (AR-V7 Specific)分子别名
Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; DHTR; NR3C4; AR免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P10275克隆号
S-2694-53抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
22Rv1纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu
The Androgen Receptor splice variant 7 (AR-V7) is a constitutively active, ligand-binding domain–deleted form of the androgen receptor that retains the N-terminal transactivation domain and DNA-binding domain, enabling ligand-independent nuclear translocation and transcriptional activation of AR target genes; its expression, driven by alternative splicing or gene rearrangements, is markedly enriched in metastatic castration-resistant prostate cancer and functions as a key driver of resistance to second-generation anti-androgens such as enzalutamide and abiraterone by sustaining AR signaling even under systemic androgen suppression or pharmacologic AR inhibition.
免疫印迹
WB result of Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb
Primary antibody: Androgen Receptor (AR-V7 Specific) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: T-47D whole cell lysate; LNCaP whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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