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宿主来源
Rabbit抗原名称
SQLE分子别名
Squalene monooxygenase; Squalene epoxidase (SE); ERG1免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
Q14534克隆号
S-2358-207抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
HEK-293, Raji, PC-3, A549, HepG2, F9, mouse testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms ICC 1:500 Hu ICFCM 1:500 Hu
SQLE (squalene epoxidase or squalene monooxygenase) is a 64 kDa, 574-residue FAD-dependent endoplasmic-reticulum enzyme that catalyzes the stereospecific epoxidation of squalene to 2,3-oxidosqualene, the first committed oxygenation and second rate-limiting step of cholesterol biosynthesis; it comprises an N-terminal cholesterol-sensing regulatory domain that undergoes MARCH6-mediated ubiquitination and proteasomal degradation when membrane cholesterol is high, and a C-terminal catalytic domain that uses NADPH-cytochrome P450 reductase to transfer electrons for the reaction, while its own substrate squalene can bind the regulatory domain to suppress degradation, thus functioning as both feed-forward signal and metabolite, and because fungal SQLE is sufficiently divergent, selective inhibitors such as terbinafine block fungal ergosterol synthesis without affecting the human enzyme, making SQLE a validated antimicrobial target.
免疫印迹
WB result of SQLE Recombinant Rabbit mAb
Primary antibody: SQLE Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Karpas-299 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Negative control: Karpas-299 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 55-64 kDaWB result of SQLE Recombinant Rabbit mAb
Primary antibody: SQLE Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labelling SQLE antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti- SQLE antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SQLE in tumor tissue.
Expression of SQLE in human tissue.
Expression of SQLE in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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