VPS18 Recombinant Rabbit mAb (S-2517-69)

Vacuolar protein sorting-associated protein 18 homolog,hVPS18,KIAA1475

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6417

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

VPS18
分子别名

Vacuolar protein sorting-associated protein 18 homolog; hVPS18; KIAA1475
免疫原

Synthetic Peptide
细胞定位

Lysosome, Endosome
Accession

Q9P253
克隆号

S-2517-69
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, 293T, K562, A549, HepG2, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
IF 1:100 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms, Rt
IF	1:100	Ms

背景介绍

VPS18 (Vacuolar Protein Sorting 18) is a conserved eukaryotic protein that serves as a core subunit of both the HOPS and CORVET tethering complexes, which mediate membrane docking and fusion events in the late endosomal and lysosomal pathways. It plays a critical role in vesicle trafficking, including endocytosis, autophagy, and lysosome biogenesis. VPS18 contains a RING-finger domain, suggesting E3 ubiquitin ligase activity, and has been implicated in viral replication, neurodegeneration, and tumor suppression through its regulation of receptor degradation and intracellular signaling pathways.

免疫印迹
- WB result of VPS18 Recombinant Rabbit mAb
  Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Lane 4: A549 whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 110 kDa
  Observed MW: 110 kDa
  This blot was developed with high sensitivity substrate
- WB result of VPS18 Recombinant Rabbit mAb
  Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 110 kDa
  Observed MW: 110 kDa
  This blot was developed with high sensitivity substrate
- WB result of VPS18 Recombinant Rabbit mAb
  Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 110 kDa
  Observed MW: 110 kDa
  This blot was developed with high sensitivity substrate
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse brain. Anti-VPS18 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of VPS18 in tumor tissue.
- Expression of VPS18 in human tissue.
- Expression of VPS18 in mouse & rat tissue.