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Rabbit抗原名称
Transgelin 2分子别名
Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homolog; KIAA0120; TAGLN2免疫原
Synthetic Peptide细胞定位
CytoskeletonAccession
P37802克隆号
S-2809-73抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A549, HeLa, K562, HEK-293, NIH/3T3, mouse spleen, rat spleen, COS-7预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:5000 Hu
Transgelin 2, also known as TAGLN2 or SM22β, is a 22-kDa actin-binding protein belonging to the calponin family, widely expressed in both smooth muscle and non-muscle cells, where it stabilizes and remodels the actin cytoskeleton by cross-linking and bundling F-actin, thereby regulating cell shape, motility, immune synapse formation, and vesicle trafficking; notably, it is markedly up-regulated during dendritic-cell maturation and T-cell activation, promotes tumor growth, invasion, and drug resistance in many cancers, yet exogenous cell-permeable transgelin-2 peptides can enhance dendritic-cell homing to lymph nodes and potentiate anti-tumor immunity, highlighting its context-dependent roles as both an oncogenic factor and a potential immunotherapeutic agent.
免疫印迹
WB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDaWB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDaWB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDaWB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDaWB result of Transgelin 2 Recombinant Rabbit mAb
Primary antibody: Transgelin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Negative control: mouse skeletal muscle lysate; mouse heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Transgelin 2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
组织表达图谱
Expression of Transgelin 2 in tumor tissue.
Expression of Transgelin 2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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