分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
PKC delta分子别名
Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; PKCD; PRKCD免疫原
Synthetic Peptide细胞定位
Mitochondrion, Cell membrane, Cytoplasm, NucleusAccession
Q05655克隆号
S-2563-26抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
Jurkat, SH-SY5Y, HeLa, NIH/3T3, mouse brain, C6, rat brain预测反应种属
(反应种属缩写表)Dg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:100 Hu, Ms
PKC delta (Protein Kinase C delta) is a serine/threonine protein kinase belonging to the Protein Kinase C family, and its activation is highly dependent on calcium ions and diacylglycerol (DAG) signaling. Unlike other PKC isoforms, PKC delta plays a dual role in cells, promoting both cell survival and apoptosis, with its specific function depending on cell type, subcellular localization, and activation context. It regulates critical cellular processes such as proliferation, differentiation, migration, and DNA damage response by participating in multiple signaling pathways (e.g., NF-κB and MAPK pathways). PKC delta is particularly important in immune regulation, cancer development, and neurological functions. Studies have shown that its abnormal expression or activation is closely associated with tumor progression, autoimmune diseases, and cardiovascular pathologies, making it a potential therapeutic target. The activity of PKC delta is finely regulated by phosphorylation, proteolytic cleavage, and changes in subcellular localization. For example, during apoptosis, it is cleaved by caspases to generate a constitutively active catalytic fragment that amplifies cell death signals.
免疫印迹
WB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDaWB result of PKC delta Recombinant Rabbit mAb
Primary antibody: PKC delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- PKC delta antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- PKC delta antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)