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Phospho-RIP (Ser166) Recombinant Rabbit mAb (S-2702-58)

Receptor-interacting serine/threonine-protein kinase 1,Cell death protein RIP,Receptor-interacting protein 1 (RIP-1),Rinp,Rip,Ripk1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6410

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-RIP (Ser166)
分子别名

Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); Rinp; Rip; Ripk1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

Q60855
克隆号

S-2702-58
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:500	Hu

背景介绍

Phospho-RIP (Ser166) is the phosphorylated form of receptor-interacting protein kinase 1 (RIPK1) at serine 166, a modification that acts as a biomarker for the kinase’s activation and marks the initiation of RIPK3/MLKL-dependent necroptotic signaling; this phosphorylation event converts RIPK1 from a pro-survival scaffold into a death-inducing kinase, enabling assembly of the necrosome complex (RIPK1–RIPK3–MLKL) that drives inflammatory, caspase-independent cell death, and its detection is therefore widely used to monitor necroptotic activity in cancer, neurodegeneration and inflammatory disease research.

免疫印迹
- WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 75 kDa
  Observed MW: 75 kDa
- WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated L-929 whole cell lysate 20 µg
  Lane 2: L-929 treated with 20 μM Z-VAD for 30 minutes, then treated with 20 ng/ml mTNF-α and 100 nM SM-164 for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 75 kDa
  Observed MW: 75 kDa
免疫沉淀
免疫细胞化学
- ICC analysis of HeLa cells treated with Calyculin A(100nM,30min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-RIP (Ser166) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).