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Rabbit抗原名称
CD115分子别名
Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; FMS; CSF1R细胞定位
Cell membraneAccession
P07333克隆号
S-3346抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu
CD115, also known as Colony-Stimulating Factor 1 Receptor (CSF1R) or M-CSF receptor (M-CSFR), is a 972-amino-acid cell-surface glycoprotein encoded by the CSF1R gene (c-FMS proto-oncogene) that belongs to the type III receptor tyrosine kinase family; it serves as a receptor for the cytokines CSF-1 and IL-34, and is highly expressed on monocyte-lineage cells and osteoclasts, mediating their survival, proliferation, differentiation, and motility through ligand-induced oligomerization and transphosphorylation, with mutations linked to myeloid malignancies and increased expression observed in Alzheimer’s disease microglia and breast carcinogenesis.
免疫印迹
WB result of Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-CSF-1R/M-CSF-R (Tyr708) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 strave overnight, then treated with 100 ng/ml hM-CSF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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