Phospho-Bad (Ser112) Recombinant Rabbit mAb (S-3596)
Bcl2-associated agonist of cell death,Bcl-2-binding component 6,Bcl-2-like protein 8 (Bcl2-L-8),Bcl-xL/Bcl-2-associated death promoter,Bcl2 antagonist of cell death,BBC6,BCL2L8,BAD
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宿主来源
Rabbit抗原名称
Phospho-Bad (Ser112)分子别名
Bcl2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-2-like protein 8 (Bcl2-L-8); Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BBC6; BCL2L8; BAD细胞定位
Cytoplasm, MitochondrionAccession
Q92934克隆号
S-3596抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt, Mk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt, Mk
Phospho-Bad (Ser112) is the phosphorylated form of the pro-apoptotic Bcl-2 family member Bad at serine 112, a modification catalyzed by survival kinases such as PKB/Akt, p90RSK or Pim-2 that creates a 14-3-3-binding motif, sequestering Bad in the cytosol and thereby preventing it from neutralizing anti-apoptotic Bcl-2/Bcl-xL at the mitochondrial outer membrane; this single phosphorylation event thus functions as a molecular switch converting Bad from an apoptosis inducer to a silent scaffold, integrating growth-factor, stress and cytokine signals to set the apoptotic threshold in neurons, lymphocytes and many cancer cells.
免疫印迹
WB result of Phospho-Bad (Ser112) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bad (Ser112) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 strave overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 20 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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