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Phospho-AMPKα (Thr172) Recombinant Rabbit mAb (S-2216-10)

5'-AMP-activated protein kinase catalytic subunit alpha,AMPK subunit alpha,Acetyl-CoA carboxylase kinase (ACACA kinase),Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase),Tau-protein kinase PRKAA1,PRKAA1,AMPK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6406

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-AMPKα (Thr172)
分子别名

5'-AMP-activated protein kinase catalytic subunit alpha; AMPK subunit alpha; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; PRKAA1; AMPK1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13131, P54646
克隆号

S-2216-10
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt, Mk
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:500-1:1000 Hu, Ms, Rt, Mk

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:500-1:1000	Hu, Ms, Rt, Mk

背景介绍

Phospho-AMPKα (Thr172) protein refers to the catalytic α-subunit of AMP-activated protein kinase that has been phosphorylated on threonine 172, a post-translational modification catalyzed primarily by the upstream kinase LKB1 (or CaMKK2 in response to calcium signals) that is essential for switching AMPK from an inactive to an active conformation; once phosphorylated, this evolutionarily conserved serine/threonine kinase acts as a master cellular energy sensor, triggering catabolic pathways such as fatty-acid oxidation and autophagy while simultaneously inhibiting energy-consuming anabolic processes like gluconeogenesis, lipogenesis and protein synthesis, thereby restoring ATP levels during metabolic stress, and its phosphorylation status is widely used as a biomarker for metabolic activity, exercise physiology, mitochondrial health and the efficacy of drugs ranging from metformin to direct AMPK activators in diabetes, cancer and cardiovascular research.

免疫印迹
- WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg (phosphatase treated membrane)
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 64 kDa
  Observed MW: 62 kDa
  This blot was developed with high sensitivity substrate
- WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg (phosphatase treated membrane)
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 64 kDa
  Observed MW: 62 kDa
  This blot was developed with high sensitivity substrate
- WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 starve overnight, then treated with 5 μM Oligomycin for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 64 kDa
  Observed MW: 62 kDa
  This blot was developed with high sensitivity substrate
斑点杂交
- Dot blot result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
  Lane 1: AMPKα (Thr172) phospho peptide
  Lane 2: AMPKα (Thr172) unmodified peptide
  Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution