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Rabbit抗原名称
p35/25分子别名
Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; CDK5R; NCK5A; CDK5R1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
Q15078克隆号
S-2719-47抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human brain, mouse brain, rat brain预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250 Hu, Ms, Rt IF 1:500 Ms, Rt
p35/25 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5): p35 is the full-length, membrane-anchored protein that rapidly turns over and keeps CDK5 activity tightly controlled, whereas p25 is the calpain-cleaved, N-terminally truncated fragment that lacks the membrane-targeting myristoylation signal, relocates CDK5 to nuclear/perinuclear compartments, and—because of its 5- to 10-fold longer half-life—induces prolonged, deregulated CDK5 hyperactivity that aberrantly phosphorylates tau and other cytoskeletal proteins, thereby contributing to neurodegeneration in Alzheimer’s disease and related tauopathies.
免疫印迹
WB result of p35/25 Recombinant Rabbit mAb
Primary antibody: p35/25 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 38, 25 kDaWB result of p35/25 Recombinant Rabbit mAb
Primary antibody: p35/25 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 38, 25 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-p35/25 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-p35/25 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-p35/25 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-p35/25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-p35/25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of P35/P25 in tumor tissue.
Expression of P35/P25 in human tissue.
Expression of P35/P25 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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