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Rabbit抗原名称
MMP14分子别名
Matrix metalloproteinase-14; MMP-14; MMP-X1; Membrane-type matrix metalloproteinase 1 (MT-MMP 1; MTMMP1); Membrane-type-1 matrix metalloproteinase (MT1-MMP; MT1MMP)免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P50281克隆号
S-2922-69抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HT-1080, BxPC-3, U-87 MG, A431纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Matrix metalloproteinase-14 (MMP14, MT1-MMP) is a trans-membrane, zinc-dependent endopeptidase that serves as a master regulator of pericellular proteolysis by cleaving virtually all extracellular-matrix proteins (collagens, laminins, fibronectin, proteoglycans), activating pro-MMP2 and pro-MMP13, shedding cell-surface receptors and adhesion molecules, and thereby governing development, angiogenesis, wound repair, adipogenesis and alveolarization, while its chronic over-expression—driven by TGF-β/SMAD, HIF-2α/Sp1 or EGF signalling and restrained by TIMP-2, microRNAs and promoter methylation—promotes tumour invasion, metastasis, fibrosis and obesity, making it a key therapeutic target in cancer, Graves’ orbitopathy, BPD and other ECM-related disorders.
免疫印迹
WB result of MMP14 Recombinant Rabbit mAb
Primary antibody: MMP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 58 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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