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Rabbit抗原名称
GPD2分子别名
Glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; GPDH-M; mitohondrial glycerophosphate dehydrogenase gene (mGDH); mtGPD免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P43304克隆号
S-2941-41抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, MCF7, U-87 MG, Caco-2, LNCaP, NIH/3T3, mouse skeletal muscle, C6, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu
GPD2 (glycerol-3-phosphate dehydrogenase 2, mitochondrial) is a 727-amino-acid, FAD-binding inner-mitochondrial-membrane enzyme that catalyzes the irreversible oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate, feeding electrons into the respiratory chain at coenzyme Q and thereby serving as the rate-limiting component of the glycerol-phosphate shuttle that re-oxidizes cytosolic NADH to sustain glycolysis; it is expressed ubiquitously, its activity modulates glucose-stimulated insulin secretion, reactive-oxygen-species generation, platelet activation and bleeding risk, and its gene (chr2q24.1) produces two transcripts encoding the same protein.
免疫印迹
WB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Lane 5: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDaWB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDaWB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDaWB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast squamous cell carcinoma. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GPD2 in tumor tissue.
Expression of GPD2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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