AMPKβ1/2 Recombinant Rabbit mAb (S-2728-26)

5'-AMP-activated protein kinase subunit beta-1/2,AMPK subunit beta-1/2,AMPKb,AMPK

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6397

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

AMPKβ1/2
分子别名

5'-AMP-activated protein kinase subunit beta-1/2; AMPK subunit beta-1/2; AMPKb; AMPK
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q9Y478, O43741
克隆号

S-2728-26
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
IHC-P 1:200 Hu, Ms
ICC 1:100 Hu, Ms
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HepG2, HeLa, A549, 293T, HEK-293, NIH/3T3, mouse brain, mouse liver, rat brain, COS-7
预测反应种属
(反应种属缩写表)

Bv, Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu, Ms
ICC	1:100	Hu, Ms

背景介绍

AMPKβ1/2 are indispensable scaffolding isoforms of the 5′-AMP-activated protein kinase (AMPK) complex that harbor a conserved carbohydrate-binding module (CBM) which docks glycogen and related oligosaccharides to allosterically sensitize the holoenzyme to cellular energy stress, while simultaneously anchoring the α-catalytic and γ-regulatory subunits through their C-terminal sequences to ensure correct heterotrimeric assembly, stable lysosomal or mitochondrial localization, and optimal phosphorylation-mediated activation by upstream kinases such as LKB1 and CaMKK2, thereby governing systemic metabolic fluxes including fatty-acid oxidation, glucose uptake, and autophagy in liver, skeletal muscle, and adipose tissue.

免疫印迹
- WB result of AMPKβ1/2 Recombinant Rabbit mAb
  Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Lane 5: HEK-293 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30, 38 kDa
- WB result of AMPKβ1/2 Recombinant Rabbit mAb
  Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Lane 3: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30, 38 kDa
- WB result of AMPKβ1/2 Recombinant Rabbit mAb
  Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30, 38 kDa
- WB result of AMPKβ1/2 Recombinant Rabbit mAb
  Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30, 38 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- AMPKβ1/2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti- AMPKβ1/2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of AMPKβ1/2 in tumor tissue.
- Expression of AMPKβ1/2 in human tissue.
- Expression of AMPKβ1/2 in mouse & rat tissue.