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TRIM63 Recombinant Rabbit mAb (S-2304-110)

E3 ubiquitin-protein ligase TRIM63,Iris RING finger protein,Muscle-specific RING finger protein 1 (MuRF-1,MuRF1),RING finger protein 28,RING-type E3 ubiquitin transferase TRIM63,Striated muscle RING zinc finger protein,Tripartite motif-containing protein 63,IRF,MURF1,RNF28,SMRZ

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6395

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

TRIM63
分子别名

E3 ubiquitin-protein ligase TRIM63; Iris RING finger protein; Muscle-specific RING finger protein 1 (MuRF-1; MuRF1); RING finger protein 28; RING-type E3 ubiquitin transferase TRIM63; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63; IRF; MURF1; RNF28; SMRZ
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q969Q1
克隆号

S-2304-110
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

mouse heart, mouse skeletal muscle, rat heart, rat skeletal muscle
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Ms, Rt
IHC-P 1:200-1:5000 Hu, Ms, Rt
IF 1:1000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000-1:5000	Ms, Rt
IHC-P	1:200-1:5000	Hu, Ms, Rt
IF	1:1000	Hu, Ms

背景介绍

TRIM63, also known as MuRF1 (Muscle RING Finger 1), is a muscle-specific E3 ubiquitin ligase encoded by the TRIM63 gene, primarily expressed in cardiac and skeletal muscle, where it plays a critical role in regulating muscle protein homeostasis by ubiquitinating sarcomeric proteins such as MYH6, MYBPC3, and cardiac troponin I for proteasomal degradation, thereby influencing muscle atrophy and hypertrophy; structurally, TRIM63 belongs to the tripartite motif (TRIM) family and contains an N-terminal RING domain, B-box, coiled-coil motifs, and a C-terminal acidic tail, and mutations in TRIM63 have been implicated in human hypertrophic cardiomyopathy due to impaired E3 ligase activity and disrupted protein degradation pathways.

免疫印迹
- WB result of TRIM63 Recombinant Rabbit mAb
  Primary antibody: TRIM63 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Lane 3: mouse skeletal muscle lysate 20 µg
  Negative control: mouse liver lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40 kDa
  Observed MW: 40 kDa
- WB result of TRIM63 Recombinant Rabbit mAb
  Primary antibody: TRIM63 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat heart lysate 20 µg
  Lane 2: rat skeletal muscle lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40 kDa
  Observed MW: 40 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-TRIM63 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-TRIM63 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human cardiac muscle. Anti- TRIM63 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human skeletal muscle. Anti- TRIM63 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse cardiac muscle. Anti- TRIM63 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse skeletal muscle. Anti- TRIM63 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of TRIM63 in human tissue.
- Expression of TRIM63 in mouse & rat tissue.