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Rabbit抗原名称
TPH-1分子别名
Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH; TPRH; TRPH; TPH1免疫原
Synthetic PeptideAccession
P17752克隆号
S-2287-143抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu预测反应种属
(反应种属缩写表)Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu
TPH-1 (tryptophan hydroxylase-1) is a 51-kDa, biopterin-dependent and rate-limiting enzyme that catalyzes the hydroxylation of L-tryptophan to 5-hydroxytryptophan, thereby initiating peripheral serotonin synthesis in enterochromaffin cells, platelets, and mast cells; it is a homodimer whose activity is regulated by phosphorylation, allosteric cAMP-dependent mechanisms, and interaction with 14-3-3 proteins, and genetic or post-translational dysregulation of TPH-1 has been implicated in gastrointestinal disorders, cardiovascular fibrosis, and mood-related diseases.
免疫印迹
WB result of TPH-1 Recombinant Rabbit mAb
Primary antibody: TPH-1 Rabbit mAb at 1/2000 dilution
Lane 1: 293T transfected with empty vector whole cell Lysate 10 µg
Lane 2: 293T transfected with human TPH-1 whole cell Lysate 10 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 48 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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