Podoplanin Recombinant Rat mAb (S-3419)

Aggrus,Glycoprotein 36 (Gp36),PA2.26 antigen,T1-alpha (T1A),GP36,PDPN

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6392

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rat
抗原名称

Podoplanin
分子别名

Aggrus; Glycoprotein 36 (Gp36); PA2.26 antigen; T1-alpha (T1A); GP36; PDPN
细胞定位

Cytoplasm, Cell membrane
Accession

Q86YL7
克隆号

S-3419
抗体类型

Rat mAb
抗体同种型

IgG2a,k
反应种属 ?

Ms
阳性样本

Mouse lung
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Ms
IHC-P 1:250 Ms
IF 1:100 Ms

背景介绍

Podoplanin is a small mucin-type O-glycosylated type-I transmembrane protein of 162 amino acids, highly expressed on lymphatic endothelium, alveolar type I cells, tumor cells and brain neurons, where its extracellular domain—bearing the platelet-aggregation-stimulating PLAG motif and sialylated O-glycans—engages CLEC-2 on platelets to trigger platelet activation, binds CD44 and galectin-8 to promote directional cell migration and invadopodia maturation, associates with ERM proteins (ezrin-radixin-moesin) to reorganize the actin cytoskeleton and drive epithelial-mesenchymal transition, and is indispensable for developmental separation of blood and lymphatic vessels, normal alveolus formation, lymphatic vessel integrity and hippocampus-dependent synaptic plasticity and memory, while its overexpression in cancers fosters invasion, metastasis and thrombotic risk, making it a key diagnostic and therapeutic target.

免疫印迹
- WB result of Podoplanin Recombinant Rat mAb
  Primary antibody: Podoplanin Recombinant Rat mAb at 1/1000 dilution
  Lane 1: mouse lung lysate 20 µg
  Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 36 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse lung. Anti-Podoplanin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse lung. Anti- Podoplanin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of Podoplanin in mouse & rat tissue.