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Rabbit抗原名称
Phospho-c-Myc (Ser62)分子别名
Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39 (bHLHe39); Proto-oncogene c-Myc; Transcription factor p64; BHLHE39; MYC细胞定位
Cytoplasm, NucleusAccession
P01106克隆号
S-3428抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
Karpas-299, HCT-116纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
Phospho-c-Myc (Ser62) is the c-Myc transcription factor phosphorylated at serine-62, an essential post-translational modification that stabilizes the protein by preventing ubiquitin-mediated degradation, thereby sustaining c-Myc’s ability to drive expression of genes governing cell-cycle progression, metabolism and apoptosis; this phosphorylation, primarily catalyzed by ERK or CDK kinases in response to mitogenic signals, is tightly balanced by de-phosphorylation at Thr58 to trigger subsequent degradation, and its dysregulation is frequently observed in cancers where elevated phospho-Ser62-c-Myc levels correlate with hyper-proliferation and poor prognosis, making it both a biomarker and a potential therapeutic target.
免疫印迹
WB result of Phospho-c-Myc (Ser62) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-c-Myc (Ser62) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Karpas-299 whole cell lysate 20 µg
Lane 2: Karpas-299 treated with 10 µM MG-132 overnight whole cell lysate 20 µg
Lane 3: untreated HCT 116 whole cell lysate 20 µg
Lane 4: HCT 116 treated with 10 µM MG-132 overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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