MFG1-E8 Recombinant Rabbit mAb (S-2530-74)

Lactadherin,Breast epithelial antigen BA46,HMFG,MFGM,Milk fat globule-EGF factor 8 (MFG-E8),SED1,MFGE8

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6389

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MFG1-E8
分子别名

Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8 (MFG-E8); SED1; MFGE8
免疫原

Synthetic Peptide
细胞定位

Secreted
Accession

Q08431
克隆号

S-2530-74
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

A549, PANC-1, human milk
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu
IHC-P 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu
IHC-P	1:1000	Hu
ICC	1:500	Hu

背景介绍

Milk fat globule-EGF factor 8 (MFG-E8), also called lactadherin, is a secreted 66–75 kDa glycoprotein composed of an N-terminal EGF-like domain containing an RGD integrin-binding motif and two C-terminal discoidin/F5-8 domains that bind phosphatidylserine, enabling it to act as a bridging molecule between apoptotic cells and phagocytes expressing αvβ3/αvβ5 integrins, thereby orchestrating efferocytosis, dampening inflammation, and exerting pleiotropic effects on vascular remodeling, skeletal-muscle lipid metabolism, myogenic differentiation, osteoclastogenesis, and tumor progression while also giving rise to the amyloidogenic peptide medin found in aged arterial walls.

免疫印迹
- WB result of MFG1-E8 Recombinant Rabbit mAb
  Primary antibody: MFG1-E8 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Daudi whole cell lysate 20 µg
  Lane 2: HL-60 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: A549 whole cell lysate 20 µg
  Lane 5: PANC-1 whole cell lysate 20 µg
  Lane 6: human milk lysate 20 µg
  Low expression control: Daudi whole cell lysate; HL-60 whole cell lysate; HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 35-48 kDa
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti- MFG1-E8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in PANC-1 cells. Anti- MFG1-E8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of MFG1-E8 in tumor tissue.
- Expression of MFG1-E8 in human tissue.