分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
FXR1分子别名
RNA-binding protein FXR1; FMR1 autosomal homolog 1; hFXR1p免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P51114抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
MCF7, HEK-293, HeLa, HepG2, A549, C2C12, mouse liver, mouse skeletal muscle, rat liver, rat skeletal muscle预测反应种属
(反应种属缩写表)Bv, Hm纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms, Rt
FXR1 (Fragile X-related protein 1) is an evolutionarily conserved RNA-binding protein that contains three KH domains and an RGG box, enabling it to recognize and regulate the stability, splicing, subcellular transport and translation of a wide spectrum of mRNAs and miRNAs; it shuttles between nucleus and cytoplasm, acts as a cytoplasmic m⁶A reader, forms functional amyloid aggregates in neurons, controls muscle and vascular smooth-muscle cytoskeleton organization, blood pressure and inflammatory transcript decay, and is indispensable for early embryogenesis, placental trophoblast migration, neuronal miRNA processing and long-term memory, while its over-expression or amplification is linked to poor prognosis in multiple cancers.
免疫印迹
WB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDaWB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDaWB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 90 kDaWB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FXR1 in tumor tissue.
Expression of FXR1 in human tissue.
Expression of FXR1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)