MBD3 Rabbit Polyclonal Antibody

Methyl-CpG-binding domain protein 3,Methyl-CpG-binding protein MBD3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6387

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MBD3
分子别名

Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

O95983
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

293T, NTERA-2, F9, C6, rat testis, COS-7
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
IHC-P 1:200 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Ms, Rt

背景介绍

MBD3 is a 32-kDa core subunit of the nuclear Mi-2/NuRD chromatin-remodeling complex that is essential for embryonic development in mice; it binds 5-methyl-cytosine DNA indirectly through a degenerate methyl-CpG-binding domain, couples ATP-dependent nucleosome sliding with histone deacetylase activity, and thereby orchestrates transcriptional repression, pluripotency exit, lineage specification, and heterochromatin formation across vertebrate genomes.

免疫印迹
- WB result of MBD3 Rabbit pAb
  Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: NTERA-2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 33 kDa
- WB result of MBD3 Rabbit pAb
  Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
  Lane 1: F9 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 33 kDa
- WB result of MBD3 Rabbit pAb
  Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 33 kDa
- WB result of MBD3 Rabbit pAb
  Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 33 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of MBD3 in mouse & rat tissue.