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Rabbit抗原名称
DHFR分子别名
Dihydrofolate reductase免疫原
Recombinant Protein细胞定位
Cytoplasm, MitochondrionAccession
P00374克隆号
S-2542-9抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
K562, HEK-293, HeLa, Jurkat, U937, Raji, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Mk IHC-P 1:250 Hu
Dihydrofolate reductase (DHFR) is a small, ~21 kDa oxidoreductase that catalyzes the NADPH-dependent reduction of 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate, thereby regenerating the essential one-carbon carrier required for de novo purine and thymidylate synthesis, making DHFR indispensable for DNA replication and cell proliferation, and its active site, containing a deeply bound nicotinamide and a flexible Met20 loop that alternates between closed and occluded conformations to control cofactor and substrate access, has been exploited for over seven decades by antifolate drugs such as methotrexate, pemetrexed, and trimethoprim that competitively block the folate-binding pocket, with differential binding kinetics and species-specific residue changes underpinning selective toxicity toward rapidly dividing cancer cells, bacteria, and parasites, while human DHFR variants (e.g., A19V, S59G, R28W) can confer resistance to antifolates, and beyond its canonical role DHFR is also implicated in oxidative stress responses, mitochondrial folate metabolism, and as a transcriptional regulator, with post-translational modifications like phosphorylation and nitrosylation modulating its stability and subcellular localization, and its expression levels serving as prognostic markers in leukemia, breast, and lung cancers, thus continuing to position DHFR as a central hub in both basic folate biochemistry and clinically relevant chemotherapy and antimicrobial strategies.
免疫印迹
WB result of DHFR Recombinant Rabbit mAb
Primary antibody: DHFR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: U937 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDaWB result of DHFR Recombinant Rabbit mAb
Primary antibody: DHFR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DHFR in tumor tissue.
Expression of DHFR in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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