LILRB4 Recombinant Rabbit mAb (S-2504-22)
Leukocyte immunoglobulin-like receptor subfamily B member 4,B4,CD85 antigen-like family member K,Immunoglobulin-like transcript 3 (ILT-3),Leukocyte immunoglobulin-like receptor 5 (LIR-5),Monocyte inhibitory receptor HM18,CD85k,ILT3,LIR5
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宿主来源
Rabbit抗原名称
LILRB4分子别名
Leukocyte immunoglobulin-like receptor subfamily B member 4; B4; CD85 antigen-like family member K; Immunoglobulin-like transcript 3 (ILT-3); Leukocyte immunoglobulin-like receptor 5 (LIR-5); Monocyte inhibitory receptor HM18; CD85k; ILT3; LIR5免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q8NHJ6克隆号
S-2504-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
THP-1, HL-60纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:500 Hu
LILRB4 (Leukocyte Immunoglobulin-Like Receptor B4), also known as ILT3, is a type I transmembrane protein with two extracellular Ig-like domains and three intracellular ITIM motifs that recruit SHP-1/2 phosphatases to dampen myeloid-cell activation; expressed chiefly on monocytes, macrophages and dendritic cells, it functions as a key immune checkpoint that maintains self-tolerance by inhibiting NF-κB/MAPK signaling, suppressing T-cell proliferation and promoting Treg/Ts differentiation, but is exploited by tumors such as AML through ligands like ApoE to enable immune evasion, leukemia invasion and poor prognosis, making it an emerging therapeutic target in cancer, autoimmunity and transplantation.
免疫印迹
WB result of LILRB4 Recombinant Rabbit mAb
Primary antibody: LILRB4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: U266B1 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 62 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human anaplastic lymphoma. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LILRB4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LILRB4 in tumor tissue.
Expression of LILRB4 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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