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AKT2 Recombinant Rabbit mAb (S-2617-6)

RAC-beta serine/threonine-protein kinase,Protein kinase Akt-2,Protein kinase B beta (PKB beta),RAC protein kinase beta (RAC-PK-beta)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6383

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

AKT2
分子别名

RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta (PKB beta); RAC protein kinase beta (RAC-PK-beta)
免疫原

Recombinant Protein
细胞定位

Nucleus, Cytoplasm
Accession

P31751
克隆号

S-2617-6
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, MCF7, HepG2, A549, Jurkat, 293T, NIH/3T3, Neuro-2a, C2C12, mouse brain, mouse heart, mouse spleen, PC-12, C6, rat brain, rat heart, rat spleen
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
ICC 1:500 Hu, Ms, Rt
ICFCM 1:50 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms, Rt
ICC	1:500	Hu, Ms, Rt
ICFCM	1:50	Hu, Ms, Rt

背景介绍

AKT2 (also known as Protein Kinase B β, PKBβ) is a 481-residue serine/threonine kinase ubiquitously expressed but enriched in insulin-responsive tissues, where it transduces signals from the PI3K pathway through dual phosphorylation at T309 by PDK1 and S474 by mTORC2 to adopt an active conformation that phosphorylates >100 substrates—key among them GSK3β, FOXO1, AS160, and mTOR—thereby orchestrating glucose uptake via GLUT4 translocation, glycogen synthesis, cell survival, proliferation, and lipid metabolism; its dysregulation through activating E17K mutations, amplifications, or loss of PTEN contributes to type 2 diabetes, metabolic syndrome, and multiple cancers, making AKT2 a pivotal therapeutic target for isoform-selective inhibitors and metabolic modulators.

免疫印迹
- WB result of AKT2 Recombinant Rabbit mAb
  Primary antibody: AKT2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: A549 whole cell lysate 20 µg
  Lane 5: Jurkat whole cell lysate 20 µg
  Lane 6: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 58 kDa
- WB result of AKT2 Recombinant Rabbit mAb
  Primary antibody: AKT2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Lane 3: C2C12 whole cell lysate 20 µg
  Lane 4: mouse brain lysate 20 µg
  Lane 5: mouse heart lysate 20 µg
  Lane 6: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 58 kDa
- WB result of AKT2 Recombinant Rabbit mAb
  Primary antibody: AKT2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Lane 3: rat brain lysate 20 µg
  Lane 4: rat heart lysate 20 µg
  Lane 5: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 58 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling AKT2 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling AKT2 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) labelling AKT2 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-AKT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-AKT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-AKT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in C6 cells. Anti-AKT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of AKT2 in tumor tissue.
- Expression of AKT2 in human tissue.
- Expression of AKT2 in mouse & rat tissue.