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pro-Caspase-1 Recombinant Rabbit mAb (S-2677-22)

CASP-1,Interleukin-1 beta convertase (IL-1BC),Interleukin-1 beta-converting enzyme (ICE,IL-1 beta-converting enzyme),p45,Il1bc,Casp1

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6382

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Caspase-1
分子别名

CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE; IL-1 beta-converting enzyme); p45; Il1bc; Casp1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P29452
克隆号

S-2677-22
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms
阳性样本

EL4, J774A.1, A20
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IHC-P 1:250 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms
IHC-P	1:250	Ms

背景介绍

Caspase-1, the canonical member of the inflammatory caspase subfamily, is synthesized as a 45 kDa zymogen that assembles into the ~700 kDa inflammasome platform via homotypic CARD–CARD interactions with ASC and NLRP proteins; proximity-induced dimerization and trans-autoproteolysis generate the active p20/p10 tetramer whose p10 subunit forms a heterodimeric active site that cleaves the tetrapeptide motifs WEHD and YVAD, liberating the pro-inflammatory cytokines IL-1β and IL-18, triggering pyroptosis through gasdermin-D pore formation, and thereby orchestrating innate immune defense, metabolic regulation, and, when dysregulated, autoinflammatory diseases such as CAPS, atherosclerosis, and Alzheimer’s pathology.

免疫印迹
- WB result of Caspase-1 Recombinant Rabbit mAb
  Primary antibody: Caspase-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: EL4 whole cell lysate 20 µg
  Lane 3: J774A.1 whole cell lysate 20 µg
  Lane 4: A20 whole cell lysate 20 µg
  Negative control: NIH/3T3 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 46 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse colon. Anti- Caspase-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat lung. Anti- Caspase-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of Caspase-1 in mouse & rat tissue.