分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
RB1CC1分子别名
RB1-inducible coiled-coil protein 1; FAK family kinase-interacting protein of 200 kDa (FIP200); KIAA0203; RBICC免疫原
Synthetic Peptide细胞定位
Nucleus, Lysosome, CytoplasmAccession
Q8TDY2克隆号
S-2367-251抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A172, Jurkat, A20, mouse testis纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:50 Hu
RB1CC1 (also known as FIP200) is a multifunctional 183-kDa scaffolding protein that nucleates the ULK1-ATG13-RB1CC1-ATG101 complex to initiate autophagosome formation in response to nutrient or mTOR signals, acts in the nucleus as a GC-rich DNA-binding transcription factor that up-regulates RB1, p16 and p21 to enforce cell-cycle checkpoints, and simultaneously controls migration, differentiation and survival by inhibiting the focal-adhesion kinases PTK2/FAK1 and PTK2B/PYK2 and by modulating TSC1, p53 and TGF-β pathways, thereby operating as a central tumor-suppressor whose loss or mis-localation is linked to aggressive breast, prostate and pancreatic cancers as well as to fibrotic and neuro-developmental disorders.
免疫印迹
WB result of RB1CC1 Recombinant Rabbit mAb
Primary antibody: RB1CC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A172 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrateWB result of RB1CC1 Recombinant Rabbit mAb
Primary antibody: RB1CC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling RB1CC1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- RB1CC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of RB1CC1 in tumor tissue.
Expression of RB1CC1 in human tissue.
Expression of RB1CC1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)