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Rabbit抗原名称
Rac1/2/3分子别名
GX; Small G protein; p21-Rac2; RAC2; Ras-like protein TC25; p21-Rac1; RAC1; TC25; p21-Rac3; RAC3免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P15153, P63000, P60763克隆号
S-2720-10抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, HUVEC, SW480, RAW264.7, NIH/3T3, C2C12, mouse brain, PC-12, C6, rat brain预测反应种属
(反应种属缩写表)Bv, GP纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt ICC 1:100 Hu, Ms, Rt
Rac1/2/3 are highly homologous Rho-family small GTPases that cycle between an inactive GDP-bound and an active GTP-bound state to act as molecular switches relaying extracellular cues to the actin cytoskeleton, thereby orchestrating cell migration, adhesion, membrane ruffling, phagocytosis, and NADPH oxidase–mediated ROS production; while Rac1 is ubiquitously expressed and essential for lamellipodium formation, Rac2 is hematopoietic-specific and predominantly regulates neutrophil ROS generation and chemotaxis, and Rac3, enriched in brain and muscle, fine-tunes neurite outgrowth and synaptic plasticity, yet all three isoforms share overlapping upstream regulators (e.g., Vav, Tiam1, P-Rex1) and downstream effectors (e.g., PAKs, WAVE, formins) and are frequently hyperactivated in cancer, immune disorders, and cardiovascular disease, making them attractive therapeutic targets.
免疫印迹
WB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDaWB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDaWB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HEK293 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW 264.7 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Rac1/2/3 in tumor tissue.
Expression of Rac1/2/3 in human tissue.
Expression of Rac1/2/3 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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