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Rabbit抗原名称
pro Caspase-1 + p10 + p12分子别名
Caspase-1; CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE; IL-1 beta-converting enzyme); p45; IL1BC; IL1BCE; CASP1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P29466克隆号
S-2307-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
THP-1, NK-92, HL-60, EL4, mouse spleen, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
Pro Caspase-1 is a 45 kDa inactive zymogen that, upon assembly of the canonical inflammasome, undergoes autoproteolysis between the p20 and p10 subunits, releasing an N-terminal CARD domain, the p20 subunit, and the p10 subunit; the resulting heterotetramer of two p20 and two p10 chains (often described as p20-p10 plus the additional p12 peptide that can remain non-covalently associated) forms the active caspase-1 protease, which subsequently cleaves pro-IL-1β and pro-IL-18 to drive inflammatory responses.
免疫印迹
WB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: NK-92 whole cell lysate 20 µg
Lane 5: HL-60 whole cell lysate 20 µg
Negative control: HCT 116, A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 11, 12, 38, 50 kDaWB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 12, 14, 38, 50 kDaWB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 12, 14, 38, 40, 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of pro Caspase-1 + p10 + p12 in tumor tissue.
Expression of pro Caspase-1 + p10 + p12 in human tissue.
Expression of pro Caspase-1 + p10 + p12 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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