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Rabbit抗原名称
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53)分子别名
HB16; ATF2; CREB2; CREBP1; ATFA; ATF7细胞定位
Cytoplasm, NucleusAccession
P17544, P15336克隆号
S-3535抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
Anisomycin treated HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:250 Hu, Ms, Rt
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) protein is the phosphorylated form of the transcription factors ATF-2 and ATF-7 at threonine 71 and 53, respectively, which enhances their transcriptional activity by promoting dimerization and DNA binding, thereby regulating stress-responsive, inflammatory, and apoptotic gene expression through the JNK and p38 MAPK signaling pathways.
免疫印迹
WB result of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 µg/ml Anisomycin for 35 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52, 54 kDa
Observed MW: 65 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 µg/ml Anisomycin for 35 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52, 54 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) in tumor tissue.
Expression of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) in human tissue.
Expression of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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