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IFT88 Recombinant Rabbit mAb (S-2187-107)

Intraflagellar transport protein 88 homolog,Recessive polycystic kidney disease protein Tg737 homolog,Tetratricopeptide repeat protein 10 (TPR repeat protein 10),TG737,TTC10

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6375

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

IFT88
分子别名

Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10 (TPR repeat protein 10); TG737; TTC10
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

Q13099
克隆号

S-2187-107
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, 293T, NIH/3T3, mouse testis, rat testis
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
IF 1:100 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
IF	1:100	Ms

背景介绍

IFT88 (intraflagellar transport protein 88) is an 833-amino-acid tetratricopeptide-repeat protein that serves as a core subunit of the IFT-B complex, powering anterograde delivery of ciliary cargo by kinesin motors, and is therefore indispensable for assembly and maintenance of motile and primary cilia; beyond this canonical ciliary role, IFT88 also localizes to centrosomes in proliferating non-ciliated cells where it binds the Rb regulator Che-1 to restrain G1-S transition, acts in mitotic spindle orientation, endocytic recycling of immune receptors, angiogenesis, and cartilage homeostasis, while mutations or depletion of IFT88 produce developmental malformations, polycystic kidney disease, skeletal defects, retinal degeneration, and tumor progression, illustrating its broad importance in both cilium-dependent and cilium-independent cellular processes.

免疫印迹
- WB result of IFT88 Recombinant Rabbit mAb
  Primary antibody: IFT88 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 90 kDa
  This blot was developed with high sensitivity substrate
- WB result of IFT88 Recombinant Rabbit mAb
  Primary antibody: IFT88 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 90 kDa
- WB result of IFT88 Recombinant Rabbit mAb
  Primary antibody: IFT88 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 90 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-IFT88 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-IFT88 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IFT88 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-IFT88 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-IFT88 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse testis. Anti- IFT88 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of IFT88 in tumor tissue.
- Expression of IFT88 in human tissue.
- Expression of IFT88 in mouse & rat tissue.