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Rabbit抗原名称
Glycogen Synthase分子别名
Glycogen [starch] synthase, muscle; Glycogen synthase 1; GYS; GYS1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P13807克隆号
S-2698-24抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HepG2, Jurkat, A204, HEK-293纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu
Glycogen Synthase (GS) is the key rate-limiting enzyme in the glycogen biosynthesis pathway, primarily responsible for catalyzing the elongation of the glycogen chain. It uses uridine diphosphate glucose (UDP-Glucose) as the activated glucose donor, adding glucose molecules via α-1,4-glycosidic bonds to a pre-existing glycogen primer chain, thereby linearly extending the glucose polymer. The activity of this enzyme is precisely regulated by both allosteric control and covalent modification (reversible phosphorylation): the insulin signaling pathway activates its activity through dephosphorylation, promoting glycogen storage; whereas stress hormones like glucagon and adrenaline cause its inactivation through phosphorylation, thereby inhibiting glycogen synthesis and mobilizing energy release. Thus, Glycogen Synthase is a central molecule in maintaining the body's glucose homeostasis, and its aberrant activity is closely associated with metabolic diseases such as Type II diabetes and insulin resistance.
免疫印迹
WB result of Glycogen Synthase Recombinant Rabbit mAb
Primary antibody: Glycogen Synthase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A204 whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Glycogen Synthase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Glycogen Synthase in tumor tissue.
Expression of Glycogen Synthase in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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